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It is important however, that sterilization with heat or superheated water not come into direct contact with the CO2 sensor. This can be controlled by sterilization between samples. Risks of contamination inside a CO2 incubator are primarily around cross contamination from fungi, viruses and bacteria. For that reason, an atomizer may be used to boost the humidity level along with a %RH sensor to control the humidity level. While a water pan is often used to improve humidity, it creates potential problems with contamination and irregular humidity levels as the door is opened and closed. Too much water in the air can result in condensation inside the CO2 sensor which will render the sensor inoperative.
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In other words, by adding additional CO2 at the right level you prevent the pH inside the cells from becoming either alkaline or acidic, which both inhibit cell growth. Here's a short video that explains the chemical reaction. The combination of H2O and CO2 results in bicarbonate (HCO3-) and H2CO3 which keeps the pH neutral, and therefore has been found to affect the growth of biological cells the least. The H2O in the cells can be turned into a carbonic acid (H2CO3) buffer by adding additional CO2. In order to culture cells under optimum conditions, the media they grow in needs to stay at neutral pH (around pH 7). Humans are most comfortable at CO2 levels at or slightly above 400 ppm (0.04%) which raises the question, why would a CO2 incubator that is used to grow tissue cultures need CO2 levels of 5 - 10%? These three numbers create the optimal conditions for biological cell growth. They are required to maintain the same conditions as inside the human body. Hopefully all of that money and planning will pay off.CO2 incubators are sealed, climate-controlled boxes used in life science laboratories to grow biological cell cultures. That approach was developed because the notion is that the overall economy is changing and new ideas are needed to develop jobs in the state. In my state, there is a government and private partnership through which people who have great ideas that can turn into products are awarded grants and are encouraged to make their ideas a viable reality. What has been fascinating to watch is how these ideas have become viewed as increasingly valuable and are encouraged by companies and even governments at time. The goal is to help those companies develop until they are strong enough to go out on their own and grow into businesses that hire a lot of people. November 24, - Some universities have taken that idea farther by offering people with such ideas the chance to form startup companies and use collegiate resources to build their companies.
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It can be great to have a university's help, but not if the end result is developing a company that will benefit a college more than the people who had the idea that formed the company and the knowledge of how to turn something into a marketable product or service. Specifically, some universities will only allow a company to set up shop and grow in the incubator if it pledges a huge percentage of any profits to the college. November 24, - I have read about some of those so-called technology incubators and have seen there are some complaints about how they function. We don't want colleges out robbing people, but certainly a university is entitled to a little compensation if it helps people who have a great idea but no money build a legitimate company. November 25, - I do agree, but don't most colleges only want a modest percentage of any profits? That kind of arrangement would help the college get something for its investment in exchange for helping a business grow.